|
KMID : 0363420090230010159
|
|
Journal of Korean Oriental Pediatrics
2009 Volume.23 No. 1 p.159 ~ p.171
|
|
|
Inhibitory Effect of Coicis Semen Extract(CSE) on Pro-inflammatory Mediatory
|
|
Yun Hye-Jin
Lee Yu-Jin
Kang Mi-Sun
Baek Jung-Han
|
|
|
Abstract
|
|
|
Objectives: This study was evaluated the effects of CSE the regulatory mechanism of NO and cytokines in the LPS-stimulated Raw 264.7 cells.
Methods: The Coicis Semen MeOH extract dissolved in EMEM for 1 hour prior to the addition of LPS(1). The cell viability was measured by MTT assay, and Nitric Oxide production was monitored by measuring the nitrite content in culture medium. The levels of cytokine and PGE2 were analyzed by sandwich immunoassays.
Results: CSE inhibited the production of NO (0.03 and 0.1 mg/ml), (0.03 and 0.1 mg/ml), (0.03 and 0.1 mg/ml), IL-6 (0.03, 0.1 mg/ml) and PGE2(0.03 and 0.1 mg/ml) in Raw 264.7 cells activated with LPS(lipopolysaccharide).
Conclusion: According to the results above, Coicis Semen can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections.
|
|
|
KEYWORD
|
|
|
Coicis Semen, LPS, , , IL-6, PGE2
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
 
|